I will analyze your dna methylation and epigenetics data
About this Gig
I analyze epigenetics data: DNA methylation (arrays or bisulfite/enzymatic sequencing), ChIP-seq, and ATAC-seq and turn it into results you can interpret and put in a paper.
For methylation data, I work with both array-based data (Illumina 450k/EPIC) and sequencing-based data (WGBS, RRBS, EM-seq), since the analysis approach differs meaningfully between the two and I'll tell you which is relevant for your data type. This typically covers quality control, normalization, differential methylation analysis between groups, and annotation of significant regions to genes or genomic features.
For ChIP-seq and ATAC-seq, the workflow covers read alignment, peak calling, and differential accessibility/binding analysis, with visualization of signal tracks and peak annotation.
In all cases, the output is meant to be interpretable: clear plots, a summary of what the significant regions or peaks actually correspond to biologically, and enough documentation of the method that it can go straight into a methods section.
Message me with your data type and study design before ordering so I can confirm scope and give you an accurate quote.
FAQ
What type of data can I provide?
You can provide raw IDAT files or processed beta/M-value matrices. The workflow will be adapted accordingly.
Do you process raw IDAT files?
Yes, preprocessing is included as part of the package.
What tools do you use?
I primarily use standard R packages such as SeSAMe, DMRcate or missMethyl
Can you handle complex experimental designs?
Yes. I can accommodate multiple conditions, covariates, and contrasts, provided they are clearly defined beforehand.
Is biological interpretation included?
Interpretation is included.
Is my data confidential?
Absolutely. All data are treated as strictly confidential and used exclusively for your project. NDA signing is available upon request.

