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I will process bulk rna seq fastq data and perform differential expression analysis
Bioinformatics and Sequencing Data Analysis
I am a doctoral student in Medical Informatics with a background in Clinical Medicine and published bioinformatics research. I process bulk RNA-seq data, single-cell FASTQ data using Cell Ranger or Ce...
About this Gig
Bulk RNA-seq processing: $8 USD per sample.
Volume pricing per order: 1-5 samples $8 each; 6-19 $7.20; 20-49 $6.40; 50-99 $5.60; 100+ $4.80. Highest eligible discount applies to all samples; no stacking.
Packages: 5 samples $40; 25 samples $160; 50 samples $280. Message for other quantities and a custom offer. Fiverr buyer fees/taxes are separate.
Workflow: FastQC/fastp, HISAT2, featureCounts, plus one DESeq2 contrast, PCA, volcano/heatmap and GO or GSEA when the study design supports them. A single sample supports upstream processing only; differential analysis requires biological replicates.
Human, mouse or rat; suitable reference required. Up to 30 million read pairs per sample. Same reference and agreed workflow per order. Deliverables: QC, tables, scripts/parameters, English methods README and one scope-fix revision.
Send sample count, assay, depth, groups and reference before ordering. Extra contrasts, special references and deeper data require a separate scope. Authorized data transfer only. Processing only; no standalone download service. Research use; no clinical interpretation or guaranteed significance.
Programming language:
R
Technology:
Other
Analysis Type:
Quantitative Analysis
•
Statistical Analysis
Expertise:
Statistics
•
Hypothesis testing
Tools:
RStudio
FAQ
What should I send before ordering?
Send organism, assay, sample count, read-pair count, reference build and sample metadata with groups and contrasts. All packages accept FASTQ. Differential analysis requires a suitable study design and biological replicates. Agree on an authorized, secure data-transfer method before ordering.
