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I will analyze fastq sequencing data and perform ngs quality control
About this Gig
I will analyze your FASTQ sequencing data and perform NGS quality control using a reproducible bioinformatics workflow.
The analysis can include:
Read and base counts
Read length statistics
GC% and N% analysis
Phred quality scores
Q20 and Q30 metrics
Quality-by-position analysis
GC and read-length distributions
Raw vs. processed quality comparison
QC visualizations and a clear results summary
I can work with paired-end FASTQ/FASTQ.GZ files and provide organized outputs that are easy to interpret and reuse.
This service is suitable for research projects, academic work, genomic data analysis, and preliminary NGS data assessment.
Please provide your FASTQ files and any relevant sample information before ordering so I can confirm that the requested analysis fits the selected package.
Programming language:
Python
•
SQL
•
Colab
•
NoSQL
Tools:
Jupyter Notebook
•
OpenCV
•
TensorFlow
•
Excel
FAQ
What type of sequencing files do you need?
I primarily work with FASTQ or FASTQ.GZ files. For paired-end sequencing, please provide both R1 and R2 files. Please also provide any available information about the sample, sequencing platform, and experimental context.
Do you analyze paired-end FASTQ data?
Yes. The service supports paired-end FASTQ data when both forward (R1) and reverse (R2) files are provided. The selected package determines the number of datasets included
What quality metrics will you analyze?
Depending on the selected package, analysis can include read and base counts, read length, GC%, N%, Phred quality scores, Q20/Q30, quality by position, GC distribution, read-length distribution, and raw-versus-processed comparisons.
Can you perform adapter and quality trimming?
Yes, when preprocessing is included in the selected package. The specific trimming workflow and parameters will depend on the sequencing data and project requirements.
Will I receive the analysis results and visualizations?
Yes. Results are provided as organized analysis outputs with relevant tables, quality metrics, visualizations, and a concise interpretation of the observed sequencing quality.
Can you analyze my data before I place an order?
Yes. Please contact me first with your FASTQ file details, dataset size, sequencing layout, and the analysis you need. I can confirm the appropriate package and scope before you order.
Can you work with FASTQ.GZ files?
Yes. Compressed FASTQ files (.fastq.gz) are supported.
Can you provide a custom bioinformatics workflow?
Yes, for requirements outside the listed packages, please contact me with your workflow requirements and dataset details. I can discuss a custom scope and provide a suitable offer.
